e. coli (test)ecoli_collection Version 1 (Collection)test upload of e coli from sbh prime
Cello E. Coli PartsCelloEColiParts_collection Version 1 (Collection)These are the Cello parts for E. Coli circuits
SBOL Compliant SoftwareSBOLCompliantSoftware_collection Version 1 (Collection)A collection of software that supports the Synthetic Biology Open Language (SBOL) standard.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCryptococcusneoformansDatabaseinSyntheticBiologyOpenLanguage_collection Version 1 (Collection)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
BBa_K1428000BBa_K1428000 Version 1 (Component)T7 promoter-driven BMC structural genes with a His6-tagged component for purification
Free Genes Feature Librariesfree_genes_feature_libraries_collection Version 1 (Collection)A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Cas9CnDatabase_Cas9 Version 1 (Component)Organism: Cryptococcus neoformans var. grubi H99
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_CnD_collection Version 1 (Collection)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_CnDatabase_collection Version 1 (Collection)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
CnU6CnDatabase_CnU6_terminator Version 1 (Component)Organism: Cryptococcus neoformans var. neoformans JEC21
GFPCnDatabase_GFP Version 1 (Component)Organism: Cryptococcus neoformans serotype A H99S
GPDCnDatabase_GenBank_AE017346_Region_940695_942557 Version 1 (Component)Organism: Cryptococcus neoformans var. neoformans JEC21
CnU6CnDatabase_GenBank_AE017348_Region_871991_872263 Version 1 (Component)Organism: Cryptococcus neoformans var. neoformans JEC21
GAL7CnDatabase_GenBank_AE017353_Region_190704_1911746 Version 1 (Component)Organism: Cryptococcus neoformans var. neoformans JEC21
URA5CnDatabase_GenBank_CM000046_Region_301512_302226 Version 1 (Component)Organism: Cryptococcus neoformans var. neoformans B-3501A
URA5CnDatabase_GenBank_CM000046_Region_302227_303005 Version 1 (Component)Organism: Cryptococcus neoformans var. neoformans B-3501A
URA5CnDatabase_GenBank_CM000046_Region_303006_303540 Version 1 (Component)Organism: Cryptococcus neoformans var. neoformans B-3501A
ACT1CnDatabase_GenBank_CP003820_Region_1242235_1243067 Version 1 (Component)Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)
TRP1CnDatabase_GenBank_CP003828_Region_1023919_1024166 Version 1 (Component)Organism: Cryptococcus neoformans var. grubi H99
HOG1CnDatabase_GenBank_CP003830_Region_176667_176966 Version 1 (Component)Organism: Cryptococcus neoformans var. grubi H99
GAL1CnDatabase_GenBank_CP003831_Region_176110_177085 Version 1 (Component)Organism: Cryptococcus neoformans var. grubii H99
UGE2CnDatabase_GenBank_CP003831_Region_176453_177430 Version 1 (Component)Organism: Cryptococcus neoformans var. grubii H99
GAL7CnDatabase_GenBank_CP003831_Region_178967_179986 Version 1 (Component)Organism: Cryptococcus neoformans var. grubii H99
TEF1CnDatabase_GenBank_CP003831_Region_375181_376053 Version 1 (Component)Organism: Cryptococcus neoformans var. grubi H99
TEF1CnDatabase_GenBank_CP003831_Region_377755_378976 Version 1 (Component)Organism: Cryptococcus neoformans var. grubi H99
APP1CnDatabase_GenBank_CP047908_Region_1244167_1244926 Version 1 (Component)Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)
HYGCnDatabase_Hygromicin_Resistance Version 1 (Component)Organism: Cryptococcus neoformans serotype A strain H99O
amdSCnDatabase_amdS Version 1 (Component)Organism: Cryptococcus neoformans serotype A strain H99O
mCherryCnDatabase_mCherry Version 1 (Component)Organism: Cryptococcus neoformans serotype A H99S strain
SBOLDesigner CAD ToolSBOL_Software_SBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Test1Test1_collection Version 1 (Collection)Test1
Free Genes Feature LibrariesFreeGenesFeatureLibraries_collection Version 1 (Collection)A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
Altered Sequence ActivitiesAlteredSequenceActivities_collection Version 1 (Collection)A set of activities to point to ways in which sequences have been altered between source and upload.
BBa_K1740000BBa_K1740000 Version 1 (Component)Dronpa 145N ORF (E. coli optimized)
IBMc050IBMc050 Version 1 (Component)pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
IBMc052IBMc052 Version 1 (Component)pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*-T
IBMc065IBMc065 Version 1 (Component)pSB3T5(BsaI-)-PBAD-B30-mCherry*(1-159)-GAG-M86N-cat-Plux2-B32-M86C-S-mCherry*(160-236)-T
IBMc071IBMc071 Version 1 (Component)pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T